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Bolt withdrew from the final of the men’s 100 metres after Friday night’s semi-finals as Yohan Blake went on to win the trial in 9.95 seconds.
“After feeling discomfort in my hamstring after the first round last night (Thursday) and then again in the semi-final tonight (Friday) I was examined by the Chief Doctor of the National Championships and diagnosed with a Grade 1 tear,” Bolt said on his official Facebook account.
“I have submitted a medical exemption to be excused from the 100m final and the remainder of the National Championships.
“I will seek treatment immediately and hope to show fitness at the London Anniversary Games on July 22 to earn selection for the Olympic Games in Rio,” the two-time Olympic 100 metre and 200 metre gold medallist added.
Bolt had clocked 10.04 seconds to win semi-final one after a poor start.
His withdrawal does not mean he would miss the Rio Olympics, where Bolt is expected to defend his 100 and 200 metre titles.
Unlike the United States, where the first three finishers in the trials win Olympic berths, Jamaica’s selection policy allows medical exemptions.
His coach Glen Mills told Reuters that efforts were made since Thursday night to get Bolt ready for Friday’s semi-final and final.
“The therapist did a good job working on him but in a couple of hours to come and run, he felt it again,” Mills said.
“Hamstring injuries are always serious. The fact that he couldn’t run, showed it is serious, but we’ll see what happens between now and (the Olympics),” Mills said at trackside after another of his charge, Blake, won the trial.